WebSep 20, 2024 · 数据采集方法具体点,1、已取得5天的结果：如果是连续每天一组数据，那么各组现已有第一天至第五天五组数据，组内比较采用重复测量方差分析两组间比较（每 … WebApr 18, 2016 · This article describes the use of cycloheximide chase followed by western blotting to specifically analyze protein degradation in the model unicellular eukaryote, …
MG132和CHX的作用 - 知乎 - 知乎专栏
WebPulse-chase analysis involves labelling of all proteins by culturing cells in the 'pulse phase' medium containing a radioactive labeled amino acid and subsequently following the proteins in 'chase ... WebWe have generated a set of dual-reporter human cell lines and devised a chase protocol to quantify proteasomal degradation of a ubiquitin fusion degradation (UFD) substrate, a ubiquitin ligase CRL2VHL substrate, and … grammarly dgist
CHX 丁香园搜索 - DXY.cn
Web放线菌酮（ cycloheximide）是一种具苦味的物质，在 真核生物 中对 蛋白质生物合成 过程有 抑制 效应的化合物，它是 灰色链霉菌 （Streptomyces griseus）的一种产物。. 它通过干扰蛋白质合成过程中的易位步骤而阻碍 转译过程 。. 在生物药学研究中放线菌酮常被用来 ... WebNov 4, 2015 · Cycloheximide (Naramycin A) 是一种抗真菌 (antifungal) 抗生素，是真核生物蛋白质合成 (protein synthesis) 的抑制剂，抑制体内蛋白质合成和 RNA 合成的 IC50 值 … WebApr 18, 2016 · This article describes the use of cycloheximide chase followed by western blotting to specifically analyze protein degradation in the model unicellular eukaryote, Saccharomyces cerevisiae (budding yeast). In this procedure, yeast cells are incubated in the presence of the translational inhibitor cycloheximide. Aliquots of cells are collected ... grammarly discord