WebDec 7, 2024 · In order to measure the GFP and DsRed fluorescence, we constructed a dedicated spectroscopy system. The system consists of sources for fluorescence excitation, a custom optical probe, and a ... WebSep 25, 2003 · The red fluorescent protein DsRed is an attractive reporter for gene expression in living cells [1]. Due to its orange-red fluorescence, DsRed can be easily distinguished from the green fluorescent protein (GFP) …
Codon-optimized DsRed fluorescent protein for use in …
WebThe DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471. Affinity purified recombinant Discosoma sp. red fluorescent protein (rDsRed-Monomer) can be used as a standard for protein gels, Western blots, and fluorometry. It can also be used as a control for ... WebWe now report the 1.9 A crystal structure of DsRed, a red fluorescent protein from Discosoma coral. DsRed monomers show similar topology to GFP, but additional chemical modification to the chromophore extends the conjugated pi-system and likely accounts for the red-shifted spectra. Oligomerization of DsRed occurs at two chemically distinct ... pinckney recreation area silver lake
A DsRed fluorescent protein marker under - Springer
WebThis novel fluorescent protein was originally called drFP583, but is now commonly known as DsRed. When expressed in cells, the fully matured DsRed fluorescent protein is optimally excited at 558 nanometers, and has an emission maximum at 583 nanometers. However, there are multiple problems associated with DsRed when used for live cell … WebFortunately, a majority of the red fluorescent proteins (including DsRed, mCherry, JRed, tdTomato, and mPlum) can be imaged with good results using either the TRITC or Texas Red filter combinations. Optimized fluorescence filter combinations for multicolor imaging of three fluorescent proteins spanning the cyan to far-red wavelength regions are ... WebOct 1, 2024 · Fluorescent proteins (FPs) have become the work horses of molecular biology and microbiology, with numerous applications. A plethora of variants of Aequorea victoria green fluorescent protein (GFP) [] and Discosoma sp red fluorescent protein (DsRed) [] are available covering almost the whole light spectrum from green to infra-red … pinckney resolution slavery