Fluorescein is a fluorophore commonly used in microscopy, in a type of dye laser as the gain medium, in forensics and serology to detect latent blood stains, and in dye tracing. Fluorescein has an absorption maximum at 494 nm and emission maximum of 512 nm (in water). The major derivatives are fluorescein isothiocyanate (FITC) and, in oligonucleotide synthesis, 6-FAM phosphoramidite. In cellular biology, the isothiocyanate derivative of fluorescein is often used to label and track cells in fluorescence … WebFluorescein-based dyes and their conjugates have several performance characteristics that may facilitate or limit use in certain applications. Fluorescein displays: A relatively high rate of photobleaching; Fluorescent signal that is sensitive to pH changes; A relatively …
Test No. 460: Fluorescein Leakage Test Method for Identifying …
WebSep 27, 2024 · I am using a SpectraMax fluorescence polarization plate reader to assay binding of a 26kD protein to a 40 base pair target with a 5’ FITC label on one strand. WebSodium fluorescein, first synthesized by von Baeyer in 1871, is a hydrocarbon that is yellow-red in color. It has a molecular weight of 376.67. It is available as 2 to 3 mL of 25% concentration or 5 mL of 10% sodium fluorescein in a sterile aqueous solution. chrome pc antigo
Does glycerol affect fluorescent polarization result? - ResearchGate
WebThe Fluorescein Isothiocyanate (FITC) Control is light-sensitive and should be stored desiccated in the dark at 2–8 C. If properly stored, the powder should be stable for at … WebSteps for establishing and optimizing an FP receptor-ligand binding assay 1. Estimate the best settings for your instrument. The following is a suggested starting point for … WebFluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission … chrome pdf 转 图片