WebJun 1, 2009 · of collagen type I (Inamed Biomaterials, Fremont, CA) in phosphate buffered saline at 37°C. for 16 h during which primary amines react with the N-hydroxysuccinimide groups to form. WebBiological Industries Inc collagen type i. Collagen Type I, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. …

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WebThe DRG neurons were acutely dissociated as described. 22 25 Dispersed neurons were suspended in F12 medium (Sigma-Aldrich; St. Louis, Missouri, USA) (pH 7.2–7.3, 10% FCS, plated on laminin/poly-d-lysine coated (60 μL/mL) (Inamed Biomaterials; Fremont, California, USA) cover slips and incubated at 95% O 2 and 5% CO 2. Two hours after plating ... WebDec 24, 2008 · No differences in the growth rates of TSP2-null and WT wound fibroblasts were observed. Wound fibroblasts (1 × 10 5 cells/ml) were mixed in a 1-mg/ml collagen (PureCol; Inamed Biomaterials, Fremont, CA) preparation (kept at 4C) and plated in 24-well dishes precoated with 1% agarose, and incubated at 37C/5% CO 2. Three hr later, the … chive door county

Biomedical Materials - an overview ScienceDirect Topics

WebJan 7, 2024 · Approximately 400 MDCK cells were suspended in 0.4 mL of ice-cold MEM (Sigma-Aldrich, St. Louis, MO, USA) containing 2.9 mg/mL collagen (PureCol; Inamed Biomaterials, Fremont, CA, USA), 10 mM ... WebRoskilde, Denmark) coated with PureCol TM ultrapure collagen (INAMED Biomaterials, Fremont, CA, USA) at 3.1 mg/mL concentration. One mL of collagen was poured into the bottles or plate wells, spread over the entire surface, and after 3 min, the excess solution was removed. The remaining collagen was dried in a thermostat at 37 °C. WebNational Center for Biotechnology Information grasshopper uses