WebDetails. RR047B. PrimeScript™ RT Reagent Kit with gDNA Eraser (Perfect Real Time) 400 Rxns. USD $1935.00. PrimeScript RT reagent Kit with gDNA Eraser is a reverse … WebThis reverse transcription reagent kit facilitates cDNA synthesis prior to analysis by real-time PCR. Designed for two-step real-time RT-PCR (also called qRT-PCR or RT-qPCR), the …
The RLCK-VND6 module coordinates secondary cell wall …
WebPrimeScript RT reagent Kit with gDNA Eraser is a reverse-transcription kit for real-time RT-PCR (RT qPCR) that includes a genomic DNA elimination reaction. cDNA synthesis from RNA can be achieved without sample loss in a rapid reaction that … WebApr 11, 2024 · Then used the EASYSpin Plus trance RNA rapid extraction kit to obtain RNA from each sample and used the PrimeScript RT Reagent kit to synthesize the cDNA of each sample. The fluorescent quantitative primer design was completed by the NCBI primer tool, and the primer sequence information is shown in Table S1 . rich famous burgers
iScript™ Reverse Transcription Supermix, 100 x 20 µl rxns, 400 µl
WebReverse Transcription Priming for Two‑step RT Reactions Two approaches are commonly utilized for two-step RT priming; oligo-dT (O4387) and random priming. The oligo-dT (O4387) method relies on hybridization of an oligodT (usually 15mer) to the poly-A tail, present on the 3’ end of the majority of mRNA molecules, to prime and selectively reverse transcribe … WebThis reverse transcription reagent kit facilitates cDNA synthesis prior to analysis by real-time PCR. Designed for two-step real-time RT-PCR (also called qRT-PCR or RT-qPCR), the … Webreverse transcription (RT) of total RNA using a 45-nt stem-loop RT primer whose 30–8nt was reverse com-plementary to the 30–8nt of the respective miRNAs tested and a PrimeScript RT reagent kit (Takara). qPCR amplification was performed with a SYBR Premix Ex Taq II kit (Takara). The PCR mixture consisted of red panda hostel